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[1]李慧莉,何芙蓉,劉鵬程,等.外源酶強化秸稈污泥混合厭氧消化條件優(yōu)化[J].中國給水排水,2020,36(15):6-12.
LI Hui-li,HE Fu-rong,LIU Peng-cheng,et al.Optimization of Anaerobic Co-digestion Conditions of Straw and Sludge Enhanced by Exogenous Enzymes[J].China Water & Wastewater,2020,36(15):6-12.點擊復(fù)制
外源酶強化秸稈污泥混合厭氧消化條件優(yōu)化
中國給水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第36卷 期數(shù): 2020年第15期 頁碼: 6-12 欄目: 出版日期: 2020-08-01
Title:Optimization of Anaerobic Co-digestion Conditions of Straw and Sludge Enhanced by Exogenous Enzymes
作者:李慧莉1，何芙蓉1，劉鵬程2，陳志強1,3，楊子顯1(1.蘭州理工大學(xué)土木工程學(xué)院，甘肅蘭州730050；2.大連市市政設(shè)計研究院有限責(zé)任公司，遼寧大連116021;3.哈爾濱工業(yè)大學(xué)環(huán)境學(xué)院城市水資源與水環(huán)境國家重點實驗室，黑龍江哈爾濱150090)
Author(s):LI Hui-li1，HE Fu-rong1，LIU Peng-cheng2，CHEN Zhi-qiang1，3，YANG Zi-xian1(1. School of Civil Engineering, Lanzhou University of Technology, Lanzhou 730050， China; 2. Dalian Municipal Design & Research Institute Co. Ltd., Dalian 116021, China; 3. State Key Laboratory of Urban Water Resources and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090， China)
關(guān)鍵詞:玉米秸稈; 市政污泥; 厭氧消化; 纖維素酶; 蛋白酶
Keywords:corn straw; municipal sludge; anaerobic digestion; cellulase; protease
摘要:為研究不同外源酶對中溫（35 ℃）混合厭氧消化過程的影響,以秸稈污泥混合物（質(zhì)量比為1 ∶2）作為底物，在最優(yōu)投加量下將蛋白酶與纖維素酶按1 ∶1、1 ∶2、2 ∶1、1 ∶3、3 ∶1等5組投加比設(shè)置實驗，分析各組產(chǎn)氣量、甲烷含量、發(fā)酵過程氨氮含量以及多糖、蛋白質(zhì)、揮發(fā)性脂肪酸（VFAs）的含量和酶活的變化情況。結(jié)果表明,中性蛋白酶∶纖維素酶為1 ∶2的酶配比甲烷產(chǎn)量最大，為373.05 mL，TS和VS去除率分別達到10%和43.5%，且無VFAs積累，可以作為秸稈污泥混合厭氧消化最佳外源酶投加量。通過HPLC對多酶聯(lián)合反應(yīng)消化液進行測定，發(fā)現(xiàn)厭氧消化產(chǎn)物中高分子物質(zhì)主要是生物聚合物和高分子質(zhì)量腐殖酸。
Abstract:Effects of exogenous enzymes on anaerobic co-digestion at medium temperature (35 ℃) were explored. In batch experiments, the mixture of straw and sludge (mass ratio=1 ∶2) was used as the substrate. Under the optimal dosage, five groups of protease and cellulase with the ratio of 1 ∶1, 1 ∶2, 2 ∶1, 1 ∶3 and 3 ∶1 were set. The gas production, methane content, ammonia nitrogen, polysaccharide, protein, volatile fatty acids (VFAs) and enzyme activity were analyzed. The maximum methane production (373.05 mL) with the TS removal efficiency of 10% and the VS removal efficiency of 43.5% was obtained when the ratio of neutral protease and cellulase was 1 ∶2, and there was no VFAs accumulation. Therefore, the enzyme ratio of 1 ∶2 could be used as the optimal dosage of exogenous enzymes for anaerobic codigestion of straw and sludge. The slurry of multi-enzyme combined reaction was determined by HPLC. It was found that the main macromolecule substances of anaerobic digestion products were biopolymer and high molecular weight humic acid.
相似文獻/References:
[1]魯濤,朱寶飛,石春梅,等.污泥碳化系統(tǒng)試運行及污泥炭回歸土壤可行性分析[J].中國給水排水,2018,34(21):108.
LU Tao,ZHU Bao fei,SHI Chun mei,et al.Test Run of Sewage Sludge Carbonization System and Feasibility Analysis of Sludge Carbon Land Use[J].China Water & Wastewater,2018,34(15):108.
[2]孫建升,葉雅麗,鄭興燦,等.昆明市污水處理廠污泥處理工藝的研究與探討[J].中國給水排水,2020,36(18):108.
SUN Jian-sheng,YE Ya-li,ZHENG Xing-can,et al.Research and Discussion on the Sludge Disposal Technology of Kunming WWTPs[J].China Water & Wastewater,2020,36(15):108.
[3]張會文,代曉炫,姜偉,等.市政污泥的水熱反應(yīng)減量化及水分賦存形態(tài)研究[J].中國給水排水,2021,37(7):96.
ZHANG Hui-wen,DAI Xiao-xuan,JIANG Wei,et al.Reduction and Water Forms of Municipal Sludge Treated by Hydrothermal Reaction[J].China Water & Wastewater,2021,37(15):96.
[4]呂開雷,楊淘,陳紫君,等.電解鋁煙氣余熱低溫干化處理市政污泥工程應(yīng)用[J].中國給水排水,2021,37(12):120.
Lü Kai-lei,YANG Tao,CHEN Zi-jun,et al.Application of Low Temperature Drying in Municipal Sludge Treatment with Waste Heat of Electrolytic Aluminum Flue Gas[J].China Water & Wastewater,2021,37(15):120.
[5]李義爍,梁遠,顏瑩瑩,等.餐廚垃圾/市政污泥/城市糞便聯(lián)合厭氧消化沼液處理設(shè)計[J].中國給水排水,2021,37(14):56.
LI Yi-shuo,LIANG Yuan,YAN Ying-ying,et al.Design of Treatment Process of Biogas Slurry from Anaerobic Co-digestion of Kitchen Waste/Municipal Sludge/Urban Excrement[J].China Water & Wastewater,2021,37(15):56.
[6]李彪,周欣,高波,等.順義區(qū)污泥干化焚燒處理工程工藝設(shè)計[J].中國給水排水,2021,37(14):63.
LI Biao,ZHOU Xin,GAO Bo,et al.Design of Sludge Drying Incineration Treatment Process in Shunyi District[J].China Water & Wastewater,2021,37(15):63.
[7]東東,趙珊,郭學(xué)彬,等.典型污泥高級厭氧消化工藝中惡臭物質(zhì)的分布特征[J].中國給水排水,2021,37(21):7.
DONG Dong,ZHAO Shan,GUO Xue-bin,et al.Distribution Characteristics of Odorants in Typical Sludge Advanced Anaerobic Digestion Process[J].China Water & Wastewater,2021,37(15):7.
[8]程文,蔣嵐嵐,耿震,等.太原市城市污水廠污泥資源化利用工程設(shè)計[J].中國給水排水,2022,38(24):72.
CHENGWen,JIANGLan-lan,GENGZhen,et al.Engineering Design of Sludge Resource Utilization in Taiyuan Municipal Wastewater Treatment Plant[J].China Water & Wastewater,2022,38(15):72.
[9]李思敏,李思雨,唐鋒兵,等.通風(fēng)速率對市政污泥好氧堆肥氮素轉(zhuǎn)化的影響[J].中國給水排水,2023,39(5):121.
LISi-min,LISi-yu,TANGFeng-bing,et al.Effect of Ventilation Rate on Nitrogen Transformation of Municipal Sludge in Aerobic Composting[J].China Water & Wastewater,2023,39(15):121.
[10]劉欣欣,龐敏,李會東,等.污泥基磁性吸附劑制備及其去除廢水中銅的研究[J].中國給水排水,2023,39(21):99.
LIUXin-xin,PANGMin,LIHui-dong,et al.Preparation of Sludge-based Magnetic Adsorbent and Its Removal of Copper from Wastewater[J].China Water & Wastewater,2023,39(15):99.
更新日期/Last Update: 2020-08-01
[1]李慧莉,何芙蓉,劉鵬程,等.外源酶強化秸稈污泥混合厭氧消化條件優(yōu)化[J].中國給水排水,2020,36(15):6-12.
LI Hui-li,HE Fu-rong,LIU Peng-cheng,et al.Optimization of Anaerobic Co-digestion Conditions of Straw and Sludge Enhanced by Exogenous Enzymes[J].China Water & Wastewater,2020,36(15):6-12.
點擊復(fù)制
外源酶強化秸稈污泥混合厭氧消化條件優(yōu)化
中國給水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第36卷 期數(shù): 2020年第15期 頁碼: 6-12 欄目: 出版日期: 2020-08-01
- Title:
- Optimization of Anaerobic Co-digestion Conditions of Straw and Sludge Enhanced by Exogenous Enzymes
- 作者:
- 李慧莉1，何芙蓉1，劉鵬程2，陳志強1,3，楊子顯1
- (1.蘭州理工大學(xué)土木工程學(xué)院，甘肅蘭州730050；2.大連市市政設(shè)計研究院有限責(zé)任公司，遼寧大連116021;3.哈爾濱工業(yè)大學(xué)環(huán)境學(xué)院城市水資源與水環(huán)境國家重點實驗室，黑龍江哈爾濱150090)
- Author(s):
- LI Hui-li1，HE Fu-rong1，LIU Peng-cheng2，CHEN Zhi-qiang1，3，YANG Zi-xian1
- (1. School of Civil Engineering, Lanzhou University of Technology, Lanzhou 730050， China; 2. Dalian Municipal Design & Research Institute Co. Ltd., Dalian 116021, China; 3. State Key Laboratory of Urban Water Resources and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090， China)
- 關(guān)鍵詞:
- 玉米秸稈; 市政污泥; 厭氧消化; 纖維素酶; 蛋白酶
- Keywords:
- corn straw; municipal sludge; anaerobic digestion; cellulase; protease
- 摘要:
- 為研究不同外源酶對中溫（35 ℃）混合厭氧消化過程的影響,以秸稈污泥混合物（質(zhì)量比為1 ∶2）作為底物，在最優(yōu)投加量下將蛋白酶與纖維素酶按1 ∶1、1 ∶2、2 ∶1、1 ∶3、3 ∶1等5組投加比設(shè)置實驗，分析各組產(chǎn)氣量、甲烷含量、發(fā)酵過程氨氮含量以及多糖、蛋白質(zhì)、揮發(fā)性脂肪酸（VFAs）的含量和酶活的變化情況。結(jié)果表明,中性蛋白酶∶纖維素酶為1 ∶2的酶配比甲烷產(chǎn)量最大，為373.05 mL，TS和VS去除率分別達到10%和43.5%，且無VFAs積累，可以作為秸稈污泥混合厭氧消化最佳外源酶投加量。通過HPLC對多酶聯(lián)合反應(yīng)消化液進行測定，發(fā)現(xiàn)厭氧消化產(chǎn)物中高分子物質(zhì)主要是生物聚合物和高分子質(zhì)量腐殖酸。
- Abstract:
- Effects of exogenous enzymes on anaerobic co-digestion at medium temperature (35 ℃) were explored. In batch experiments, the mixture of straw and sludge (mass ratio=1 ∶2) was used as the substrate. Under the optimal dosage, five groups of protease and cellulase with the ratio of 1 ∶1, 1 ∶2, 2 ∶1, 1 ∶3 and 3 ∶1 were set. The gas production, methane content, ammonia nitrogen, polysaccharide, protein, volatile fatty acids (VFAs) and enzyme activity were analyzed. The maximum methane production (373.05 mL) with the TS removal efficiency of 10% and the VS removal efficiency of 43.5% was obtained when the ratio of neutral protease and cellulase was 1 ∶2, and there was no VFAs accumulation. Therefore, the enzyme ratio of 1 ∶2 could be used as the optimal dosage of exogenous enzymes for anaerobic codigestion of straw and sludge. The slurry of multi-enzyme combined reaction was determined by HPLC. It was found that the main macromolecule substances of anaerobic digestion products were biopolymer and high molecular weight humic acid.
相似文獻/References:
[1]魯濤,朱寶飛,石春梅,等.污泥碳化系統(tǒng)試運行及污泥炭回歸土壤可行性分析[J].中國給水排水,2018,34(21):108.
LU Tao,ZHU Bao fei,SHI Chun mei,et al.Test Run of Sewage Sludge Carbonization System and Feasibility Analysis of Sludge Carbon Land Use[J].China Water & Wastewater,2018,34(15):108.
[2]孫建升,葉雅麗,鄭興燦,等.昆明市污水處理廠污泥處理工藝的研究與探討[J].中國給水排水,2020,36(18):108.
SUN Jian-sheng,YE Ya-li,ZHENG Xing-can,et al.Research and Discussion on the Sludge Disposal Technology of Kunming WWTPs[J].China Water & Wastewater,2020,36(15):108.
[3]張會文,代曉炫,姜偉,等.市政污泥的水熱反應(yīng)減量化及水分賦存形態(tài)研究[J].中國給水排水,2021,37(7):96.
ZHANG Hui-wen,DAI Xiao-xuan,JIANG Wei,et al.Reduction and Water Forms of Municipal Sludge Treated by Hydrothermal Reaction[J].China Water & Wastewater,2021,37(15):96.
[4]呂開雷,楊淘,陳紫君,等.電解鋁煙氣余熱低溫干化處理市政污泥工程應(yīng)用[J].中國給水排水,2021,37(12):120.
Lü Kai-lei,YANG Tao,CHEN Zi-jun,et al.Application of Low Temperature Drying in Municipal Sludge Treatment with Waste Heat of Electrolytic Aluminum Flue Gas[J].China Water & Wastewater,2021,37(15):120.
[5]李義爍,梁遠,顏瑩瑩,等.餐廚垃圾/市政污泥/城市糞便聯(lián)合厭氧消化沼液處理設(shè)計[J].中國給水排水,2021,37(14):56.
LI Yi-shuo,LIANG Yuan,YAN Ying-ying,et al.Design of Treatment Process of Biogas Slurry from Anaerobic Co-digestion of Kitchen Waste/Municipal Sludge/Urban Excrement[J].China Water & Wastewater,2021,37(15):56.
[6]李彪,周欣,高波,等.順義區(qū)污泥干化焚燒處理工程工藝設(shè)計[J].中國給水排水,2021,37(14):63.
LI Biao,ZHOU Xin,GAO Bo,et al.Design of Sludge Drying Incineration Treatment Process in Shunyi District[J].China Water & Wastewater,2021,37(15):63.
[7]東東,趙珊,郭學(xué)彬,等.典型污泥高級厭氧消化工藝中惡臭物質(zhì)的分布特征[J].中國給水排水,2021,37(21):7.
DONG Dong,ZHAO Shan,GUO Xue-bin,et al.Distribution Characteristics of Odorants in Typical Sludge Advanced Anaerobic Digestion Process[J].China Water & Wastewater,2021,37(15):7.
[8]程文,蔣嵐嵐,耿震,等.太原市城市污水廠污泥資源化利用工程設(shè)計[J].中國給水排水,2022,38(24):72.
CHENGWen,JIANGLan-lan,GENGZhen,et al.Engineering Design of Sludge Resource Utilization in Taiyuan Municipal Wastewater Treatment Plant[J].China Water & Wastewater,2022,38(15):72.
[9]李思敏,李思雨,唐鋒兵,等.通風(fēng)速率對市政污泥好氧堆肥氮素轉(zhuǎn)化的影響[J].中國給水排水,2023,39(5):121.
LISi-min,LISi-yu,TANGFeng-bing,et al.Effect of Ventilation Rate on Nitrogen Transformation of Municipal Sludge in Aerobic Composting[J].China Water & Wastewater,2023,39(15):121.
[10]劉欣欣,龐敏,李會東,等.污泥基磁性吸附劑制備及其去除廢水中銅的研究[J].中國給水排水,2023,39(21):99.
LIUXin-xin,PANGMin,LIHui-dong,et al.Preparation of Sludge-based Magnetic Adsorbent and Its Removal of Copper from Wastewater[J].China Water & Wastewater,2023,39(15):99.
更新日期/Last Update: 2020-08-01
